Reconstitution of caspase-deficient MEFs with caspase-3 or caspase-7 rescues the WT phenotype. (A) Caspase-deficient MEFs were reconstituted with the appropriate caspase by retroviral transduction and caspase expression was determined by western blot. (B) WT, Casp3-/- pBabe and Casp3-/- C3 MEFs were withdrawn from serum for 4 days. At the indicated time points, cell death was determined by Annexin V-FITC staining. Data are presented as the mean ± SEM of at least 3 independent experiments. (C) WT, Casp7-/- pBabe and Casp7-/- C7 MEFs were withdrawn from serum for 4 days. At the indicated time points, percent detachment was determined by separating non-adherent from adherent cells and counting with a hemocytometer. Data are presented as the mean ± SEM of at least 3 independent experiments.
Brentnall et al. BMC Cell Biology 2013 14:32 doi:10.1186/1471-2121-14-32