Caspase-7 and-3 have distinct functions during apoptosis. (A-B) WT, Casp7-/-, Casp3-/- and Casp3-/-7-/- MEFs were withdrawn from serum for 4 days. (A) At the indicated time points, cell death was determined by Annexin V-FITC staining. (B) At the indicated time points, percent detachment was determined by separating non-adherent from adherent cells and counting with a hemocytometer. Data are presented as the mean ± SEM of at least 3 independent experiments. (C) Casp7−/− MEFs were grown on glass coverslips for 24 hr and then were washed with PBS and the medium was replaced with full medium or serum free medium for 48 hr. After, cells were fixed, stained for actin and DNA and visualized by fluorescent microscopy.
Brentnall et al. BMC Cell Biology 2013 14:32 doi:10.1186/1471-2121-14-32