Additional file 4: Figure S4.

Cytoplasmic calcium increase is required for insulin secretion under glucolipotoxic condition. Rat islets were cultured under normal conditions (control) or with 16.7 mM glucose and 500 μM palmitate for 72 h (GL). Post 72 h, islets were treated with 11 mM glucose (HG) alone or with inhibitors (5 μM Nitrendipine and 10 μM 2-APB) for 2 h and secreted insulin was measured as described in the materials and methods. Data are expressed as mean±SEM and statistical analysis was performed using the unpaired Student’s t-test. (*P<0.05, **P<0.01 and ***P<0.001, n=4).

Format: PDF Size: 5KB Download file

This file can be viewed with: Adobe Acrobat Reader

Somesh et al. BMC Cell Biology 2013 14:31   doi:10.1186/1471-2121-14-31