Additional file 1: Figure S1.
Chronic glucolipotoxic conditions increase inflammatory cytokines gene expression and cellular stress in pancreatic β-cells. Rat islets or NIT1 cells were cultured under normal conditions (control) or with 16.7 mM glucose and 500 μM palmitate for 72 h (GL). Post 72 h, RNA was isolated from islets and the mRNA levels of IL1b (A) and Nos2a (B) were measured as described in the materials and methods. Both ROS (C) and nitric oxide (D) levels were estimated in NIT1 cells as described in the Additional file 6. Data are expressed as mean±SEM and statistical analysis was performed using the unpaired Student’s t-test. (*P<0.05, **P<0.01 and ***P<0.001, n=4).
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Somesh et al. BMC Cell Biology 2013 14:31 doi:10.1186/1471-2121-14-31