Representative 3D images displaying examples of telomeric associations between heterologous chromosomes. 3D FISH with pools of telomeric probes on structurally preserved neutrophil nuclei; maximum intensity projections of confocal image stacks are shown. p telomeres are labelled in red with Alexa 568, q telomeres in green with Alexa 488 and DNA was counterstained in blue with DAPI. Pools of p and q telomeric probes for (a) the 5 submetacentric chromosomes (SSC1-SSC2-SSC3-SSC4-SSC5), (b) the 2 subtelocentric chromosomes (SSC6-SSC7), (c) the 5 metacentric chromosomes (SSC8-SSC9-SSC10-SSC11-SSC12), (d) the 6 telocentric chromosomes (SSC13-SSC14-SSC15-SSC16-SSC17-SSC18). White arrows point to pq associations.
Mompart et al. BMC Cell Biology 2013 14:30 doi:10.1186/1471-2121-14-30