Figure 2.

Representative 3D images displaying examples of telomeric associations. 3D FISH experiments were carried out with p and q telomeric probes on structurally preserved neutrophil nuclei; maximum intensity projections of confocal image stacks are shown. p telomeres are labelled in red with Alexa 568, q telomeres in green with Alexa 488 and chromosome painting probes in pink with Alexa 633. DNA was counterstained in blue with DAPI. Examples of nuclei with: (a) no association, (b) qq association, (c) pp association, (d) pp + qq associations, (e) 1- one pqloop association 2- same nucleus after addition of the chromosome painting probe, (f) 1- one pqcross association 2- same nucleus after addition of the chromosome painting probe, (g) 1- two pqloop associations, 2- same nucleus after addition of the chromosome painting probe, (h) 1- one pqloop + one pqcross associations, 2- same nucleus after addition of the chromosome painting probe, (i) example of extension of one chromosome territory on two separated lobes: one q telomere is far from its home territory. 1- nucleus, 2- chromosome 1 painting probe combined with p and q subtelomeric probes, 3- merged image.

Mompart et al. BMC Cell Biology 2013 14:30   doi:10.1186/1471-2121-14-30
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