Open Access Research article

3D organization of telomeres in porcine neutrophils and analysis of LPS-activation effect

Florence Mompart12, David Robelin12, Chantal Delcros12 and Martine Yerle-Bouissou12*

Author Affiliations

1 INRA, UMR 444, Génétique Cellulaire, F-31326 Castanet, Tolosan, France

2 Université de Toulouse, INP, ENVT, UMR 444, Génétique Cellulaire, F-31076, Toulouse, France

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BMC Cell Biology 2013, 14:30  doi:10.1186/1471-2121-14-30

Published: 26 June 2013

Additional files

Additional file 1: Table S1:

List of BAC clones selected to specifically label the p and q telomeres of each porcine chromosome.

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Additional file 2: Figure S1:

Specificity controls of probes by 2D-FISH on porcine metaphases.

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Additional file 3: Figure S2:

Labelling of the nuclear border with anti-lamin B.

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Additional file 4: Table S2:

3D distances between nuclear outer border and telomeric (p/q) spot centres in resting and LPS-activated neutrophils.

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Additional file 5: Figure S3:

Analysis of the correlation between chromosome size (in percentage of total genome length) and percentage of nuclei harbouring at least one telomeric association.

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Additional file 6: Table S3:

Probabilities of telomeric associations.

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Additional file 7: Table S4:

Analysis of telomeric associations in pool of heterologous chromosomes.

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Additional file 8: Figure S4:

Estimation of the number of events (telomeric associations) that can occur in a neutrophil nucleus.

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