Table 2

Primers used for PCR amplification to construct EGFP-mTOR and mTOR-EGFP (5' – 3' direction; restriction sites underlined)
1_HindIII_FP GGG AAA TAC GTA
    AAG CTT
AAG ATG GGG CTT GGA ACC GG
4_SalI_RP GGG CGG CCG C
    GT CGA C
CC CCA GAA AGG GCA CCA GC
5_HindIII_FP GGG AAA TAC GTA
    AAG CTT
TAC TTG GAA CCG GAC CTG C
8_SalI_RP GGG
    GTC GAC
TTA CCA GAA AGG GCA CCA GC

Yadav et al.

Yadav et al. BMC Cell Biology 2013 14:3   doi:10.1186/1471-2121-14-3

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