Table 1

Primers used for PCR amplification to construct EGFP-Rheb and DsRed-Rheb (5' – 3' direction; restriction sites underlined)
Rheb_EcoRI_FP T
    GA ATTC
GC CGC AGT CCA AGT CCC GG
Rheb_SalI_RP GGG
    GTC GAC
TCA CAT CAC CGA GCA TGA AGA C
DsRed(m)_AgeI_FP A
    AC CGG T
CG CCA CCA TGG AC
DsRed(m)_BspeI_RP GG
    T CCG GA
C TGG GAG CCG GAG TGG CG

Yadav et al.

Yadav et al. BMC Cell Biology 2013 14:3   doi:10.1186/1471-2121-14-3

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