Fluorescence lifetime imaging of EGFP-mTOR expressed in HEK293 cells. A) Confocal image of EGFP-mTOR expressed in HEK293 cells following 48 h of transfection; B) Lifetime image of the same cells (colour coding for the lifetimes is shown in (C)), image size 120 x 110 μm; C) Lifetime distribution taken for the entire area (white border) shown in (B). The data collection time for lifetime images were optimally 3 accumulations of 30 sec. with an image dimension of 128×128 pixels (the blue cross lines are for single pixel lifetime values which were not used). A fluorescence lifetime value was determined from the distribution centre in (C), representing a value for the entire area shown in (B) and was ~2450 ± 100 ps, taken from three independent experiments.
Yadav et al. BMC Cell Biology 2013 14:3 doi:10.1186/1471-2121-14-3