EGFP-mTOR expresses predominantly in the cytoplasm of mammalian cells. EGFP tagged mTOR expression was studied in HEK293, HeLa and CHO cells. The cells were transiently transfected with EGFP tagged mTOR and expression was analyzed in live cells following 48h of transfection using a Nikon TE2000 U inverted microscope and EC1 confocal system. (EGFP-mTOR; EGFP tagged at the N-terminal of mTOR and mTOR-EGFP; EGFP tagged at the C-terminal of mTOR). Bar 8 μm.
Yadav et al. BMC Cell Biology 2013 14:3 doi:10.1186/1471-2121-14-3