Additional file 14: Figure S14.

Caspases are not activated in proteasome or Vhl RNAi or sima overexpressing cells. A-H) No active caspase 3 immunoreactivity is detected in Prosα1 (A), Prosα5 (B), Prosβ2 (C), Rpt1 (D) and Rpn2 (E) RNAi fat body cells. (F) Similarly, no active caspase 3 immunolabeling is detected in Rpt1 RNAi cells (marked by Lamp1-GFP) in brains. Note that several control cells positive for active caspase 3 (arrowheads) are seen in this panel. Similarly, overexpression of sima (G) or depletion of Vhl (H) does not lead to activation of caspase 3 in fat body cells either. Boxed areas in A-H are shown enlarged. Scale bar in A equals 20 μm for A-H.

Format: PDF Size: 355KB Download file

This file can be viewed with: Adobe Acrobat Reader

Lőw et al. BMC Cell Biology 2013 14:29   doi:10.1186/1471-2121-14-29