Additional file 10: Figure S10.
Autophagic flux is enhanced upon genetic inactivation of the proteasome. A-H) Knockdown of Prosα1 (A, B), Prosα5 (C, D), Prosβ2 (E, F), and Rpt1 (G, H) cells expressing the tandemly tagged mCherry-GFP-Atg8a reporter induces the formation of mCherry-labeled autolysosomes in fat body cell clones in well fed larvae (A, C, E, G). The lysosome inhibitor chloroquine blocks autophagy-dependent quenching of GFP, as now most puncta are positive for both mCherry and GFP (B, D, F, H). Boxed areas in A-H are shown enlarged. Scale bar in A equals 20 μm for A-H.
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Lőw et al. BMC Cell Biology 2013 14:29 doi:10.1186/1471-2121-14-29