Figure 7.

ChIP analysis of WT1 binding to podxl and nphs1 promoter regions. (A) WT1 binds cis regions of podxl gene promoter in HGEC. Formaldehyde-crosslinked chromatin fragments were precipitated with either anti-WT1 antibody, anti-RNA polymerase II or no antibody as mock. Precipitated products were amplified by real-time PCR using primers for podxl proximal promoter. As normalizer, a DNA fragment lacking any WT1 site, located in the promoter region of GAPDH gene, was used. (B) WT1 binds cis regions of nphs1 gene promoter in HGEC. Formaldehyde-crosslinked chromatin fragments were precipitated with either anti-WT1 antibody, anti-RNA polymerase II or no antibody as mock. Precipitated products were amplified by real-time PCR using primers for nphs1 promoter. As normalizer, a DNA fragment lacking any WT1 site, located in the promoter region of GAPDH gene, was used. (*p<0.05 vs. HGEC:5 mM, #p<0.05 vs. HGEC:25 mM).

Tsotakos et al. BMC Cell Biology 2013 14:28   doi:10.1186/1471-2121-14-28
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