Open Access Research article

Glucose-induced gradual phenotypic modulation of cultured human glomerular epithelial cells may be independent of Wilms’ tumor 1 (WT1)

Nikolaos E Tsotakos, Marina Sagnou, Eleni S Kotsopoulou, Effie C Tsilibary and Garyfalia I Drossopoulou*

Author Affiliations

Institute of Biosciences and Applications, National Centre for Scientific Research “Demokritos”, Athens, Greece

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BMC Cell Biology 2013, 14:28  doi:10.1186/1471-2121-14-28

Published: 14 June 2013

Additional files

Additional file 1: Figure S1:

CD2AP and F-actin colocalization in podocytes. Confocal microscopic analysis for the distribution of CD2AP and F-actin in HGEC. Cells were fixed and stained with Texas Red Phalloidin (red) and anti-CD2AP antibody.

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Additional file 2: Figure S2:

α3 β1-integrin expression in podocytes. (A) Western blot analysis for α3 β1-integrin expression in HGEC. Blots were reprobed with anti-tubulin antibody, to verify protein loads, against which the data were normalized. (B) Results were expressed as the mean ± SD of three independent experiments (*p<0.05 vs. HGEC:5 mM, #p<0.05 vs. HGEC:25 mM).

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