The post-translational modification pattern and cellular localization of new isoforms. A.The post-translational modification of isoforms in LC3B, GABARAP, and GABARAPL1. 24 hours after transfection, cell lysates of HeLa cells were subjected to SDS-PAGE followed by western blotting with anti-myc and anti-his antibodies. Molecular weights (kDa) is shown on the left. B. GFP tagged LC3B, LC3B-a, GABARAP, GABARAP-a, GABARAPL1, or GABARAPL1-a were stably expressed in HeLa cells. After treated with or without starvation for 2 hours, cells were subjected to immunostaining with p62 protein as autophagosome marker. B, LC3B; B-a, LC3B-a; G, GABARAP; G-a, GABARAP-a; L1, GABARAPL1; L1-a, GABARAPL1-a. Bar: 20 μm.
Liu et al. BMC Cell Biology 2013 14:27 doi:10.1186/1471-2121-14-27