Alternative splicing patterns and expressions of human Atg8 homologs. A. Schematic diagram of the alternative splicing patterns of LC3B, GABARAP and GABARAPL1 mRNA. White and black boxes represent the protein-coding or non-coding region, respectively. Straight lines represent introns. Nucleotide sequences at the intron 3 splicing sites are shown. Splicing acceptors are underlined. Arrows show the positions of the corresponding allele specific primers. B. Expression of each transcript in multiple human tissues. Semi-quantitative PCR for each transcript was performed on human multiple-tissue cDNA panels. The expression of housekeeping gene B2M was analysed as loading control. B, LC3B; B-a, LC3B-a; G, GABARAP; G-a, GABARAP-a; L1, GABARAPL1; L1-a, GABARAPL1-a.
Liu et al. BMC Cell Biology 2013 14:27 doi:10.1186/1471-2121-14-27