Effect of syndecan-2 blockade on adhesion and migration of HCT-116 cells to stromal fibroblast ECM. (A) HCT-116 cells, previously grown on top of fibroblast ECM for three days, were incubated with anti-syndecan-2 antibody (Anti-Syn-2), IgG (IgG) or no antibodies (Fibrob. ECM) and then plated on stromal ECM-coated Petri dishes for different lengths of time. Non-adherent cells were removed, and adhesion was measured by MTT colorimetric assay. The formazan crystals formed were solubilized with DMSO, and the absorbance was measured at 540 nm. (B) Transwell membranes (Costar, Corning, 8-μm pore size) were coated with stromal fibroblast ECM as described above. HCT-116 were pre-incubated with anti-syndecan-2 antibody (Anti-Syn-2), IgG (IgG) or no antibodies (Ctrl) and plated in the top of chamber. Migrating cells were fixed in formaldehyde, stained with crystal Violet, and counted. * p ≤ 0.05.
Vicente et al. BMC Cell Biology 2013 14:25 doi:10.1186/1471-2121-14-25