Effects of high glucose and the NOS inhibitor L-NAME on the average membrane stiffness of the whole cell. Measurement of average membrane stiffness was performed after seven days of incubating endothelial cells in various media (as described in Figure 2). The average membrane stiffness of five cells in each group was assessed through the slope of the ascending part of 300 force-distance curves detected using AFM. Data shown in the figure are mean ± SD. “*”, P<0.05, compared to the control group. “#”, P<0.05, compared to the constant high glucose group.
Chen et al. BMC Cell Biology 2013 14:22 doi:10.1186/1471-2121-14-22