Effects of high glucose and the NOS inhibitor L-NAME on the expression of eNOS in human umbilical vein endothelial cells. The detection of the protein expression was carried out seven days after incubation of endothelial cells in various media (as described in Figure 2). (A) Representative western immunoblots of eNOS and β-actin. (B) Relative expression levels of eNOS proteins obtained using densitometric analyses of the blots, compared with those of β-actin. Data shown in the figure are mean ± SD. “*”, P<0.05, compared to the control group. “#”, P<0.05, compared to the constant high glucose group.
Chen et al. BMC Cell Biology 2013 14:22 doi:10.1186/1471-2121-14-22