O-FISH analyses of RNA conformational rearrangements during early HIV-1 infection. MT2 cells (a) and Jurkat cells (b) were either mock-infected or infected with HIV-1 and then fixed onto glass slides at 0, 2 or 4 hrs post-infection and incubated with a HIV-1 O-FISH probe to detect plus strand U5/PBS RNA(see Figure 2 for relative genome position). Images were analysed using Imaris software. Data shown are mean (+/− SEM) signals per cell, derived from a minimum of 5 images per condition. O-FISH signals were counted in a total of 160 cells (61 cells over 29 fields for the MT2 cells and 91 cells over 20 fields for the Jurkat cells).
Jones et al. BMC Cell Biology 2013 14:21 doi:10.1186/1471-2121-14-21