O-FISH analyses of viral replicative cDNA after blocking with AZT reverse transcriptase inhibitor. MT2 cells were infected with HIV-1 in the absence or presence of AZT at 0.1 μg/ml or 10 μg/ml concentration. Cells were fixed onto glass slides at either 20 min or 12 h post-infection and subjected to the O-FISH protocol using probes to detect both strong stop cDNA and gag cDNA. The O-FISH signals were compared to the respective AZT-untreated controls at 12 hrs post-infection. Images were analysed using Imaris software. Data shown are mean (+/− SEM) signals per cell, derived from a minimum of 5 images per condition. O-FISH signals were counted in a total of 158 cells (91 cells over 29 fields for the gag probe and 67 cells over 29 fields for the strong stop probe).
Jones et al. BMC Cell Biology 2013 14:21 doi:10.1186/1471-2121-14-21