Real-time PCR analysis of osteogenic lineage marker expression in differentiated TwiASCs and WtASCs. TwiASCs and WtASCs were induced for osteogenic differentiation. On day 7, 14 and 21, the cells were harvested and total RNA was extracted for PCR analysis of osteogenic markers ALP, RUNX and OCN. # means P< 0.05 vs WtASCs on day 7 and * means P< 0.05 vs WtASCs on day 21 for ALP, RUNX and OCN respectively (t-test, n=6).
Zhang et al. BMC Cell Biology 2013 14:20 doi:10.1186/1471-2121-14-20