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Integrin-mediated internalization of Staphylococcus aureus does not require vinculin

Marina Borisova1, Yong Shi1, Alexander Buntru12, Susanne Wörner1, Wolfgang H Ziegler3 and Christof R Hauck12*

Author Affiliations

1 Lehrstuhl Zellbiologie, Universität Konstanz, Postfach X908, 78457, Konstanz, Germany

2 Konstanz Research School Chemical Biology, Universität Konstanz, 78457, Konstanz, Germany

3 Hannover Medical School, Dept. of Paediatric Kidney, Liver and Metabolic Diseases, 30625, Hannover, Germany

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BMC Cell Biology 2013, 14:2  doi:10.1186/1471-2121-14-2

Published: 7 January 2013

Additional files

Additional file 1: Figure S1:

Similar distribution of active β1 integrins in vinculin -/- and vinculin WT cells. Vinculin WT and vinculin -/- cells were seeded on Fn coated glass bottom imaging dishes, next day fixed and stained for vinculin using mouse α-human vinculin (hVIN1) antibody, combined with biotin-SP-conjugated goat α-mouse IgG and streptavidin-FITC. In addition, integrin β1 in the ligand-bound, active conformation was detected by rat monoclonal integrin β1 antibody (clone 9EG7) together with rhodamine red conjugated goat-α-rat IgG antibody. TIRF microscopy was used to assess the distribution of vinculin and active β1 integrins. Bars represent 10 μm.

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