Wound healing response to peptide is specific for collagen. Normal human mammary epithelial (16N) as well as breast (T47D) and ovarian (OVCAR3) tumor cell monolayers, grown on 30 µg/ml fibronectin-coated glass chamber slides, were scratched and the size of the gap remaining at 4 (16N) or 24 (T47D, OVCAR3) hours was measured using SlideBook software. The size of the gap was compared between non-treated cells (white bars) and cells treated with 400 µM mimic peptide. Mean ± s.e.m., n=4 wells for each treatment (20 measurements taken for each well) *p < 0.05 vs. non-treated.
Webb et al. BMC Cell Biology 2013 14:19 doi:10.1186/1471-2121-14-19