Disruption of wound healing by the peptide is seen only in the presence of collagen. Representative confocal microscopy images of DAPI (nuclei) staining of normal 16N human mammary epithelial cells and T47D and 21MT1 breast tumor cells, grown on 3.5 µg/cm2 Cell-Tak-coated slides (A) at 4-24 hours post scratch treatment with control medium (top panel of A) or media with the 400 µM DFYNP claudin mimic peptide (bottom panel of A). The size of the gap at 4 (normal cells: 16N, EpH4) or 24 (tumor cells: T47D, 21MT1, MCF-7, OVCAR3) hours post scratch was measured, using SlideBook software, for each cell line for each condition. Mean ± s.e.m., n=3 wells for each treatment (20 measurements taken for each well) *p < 0.05 vs. non-treated.
Webb et al. BMC Cell Biology 2013 14:19 doi:10.1186/1471-2121-14-19