Peptide with glycine substitution does not inhibit cell motility. T47D cells were grown to confluence on 100 µg/ml collagen type I + 3.5 µg/cm2 Cell-Tak-coated slides. After the cells were scratched medium was replaced with fresh medium minus peptide (white bar), medium plus 400 µM DFYNP peptide (“+P”, black bar), or medium plus 400 µM DFgNP peptide (“+gP”, gray bar). Mean ± s.e.m., n=4 wells for each treatment (20 measurements taken for each well) *p < 0.05 vs. non-treated.
Webb et al. BMC Cell Biology 2013 14:19 doi:10.1186/1471-2121-14-19