Claudin-4 localization in normal and tumor cells. Representative confocal microscopy images of fixed subconfluent primary mammary epithelial cells (A) and breast cancer 21PT cells (B) treated with antibodies directed to claudin-4 (A: red, B: green) and/or ZO-1 (A: green) and stained with DAPI (A&B: blue) and/or phalloidin (B: red). Claudin-4 localizes within distinct puncta in the cytosol near the nucleus as well as along cellular projections. Arrows point to claudin-4 puncta in cellular projections in both normal and tumor cells. Yellow color of the merged image (A) indicates overlay of the red claudin and the green ZO-1 immunofluorescence.
Webb et al. BMC Cell Biology 2013 14:19 doi:10.1186/1471-2121-14-19