Table 1

Peptides identified by Orbitrap Velos mass spectrometry for PPP1R2, PPP1R2P3 or both and for PPP1CC2, after immunoprecipitation of human sperm samples with sheep or rabbit anti-PPP1R2 antibodies. aa, amino acids; pI, isoelectric point
Protein name Uniprot ID MW (Da) pI Protein size (aa) Coverage Mascot score
PPP1R2 IPP2_HUMAN 23.015 4.64 205 22.0% 74.12
Peptide Range (start-end) Number of spectra m/z meas. z Mascot score
K.WDEMNILATYHPADKDYGLMK.I 47–67 3 848.39 3 47.40
K.TSTTSSMVASAEQPRGNVDEELSK.K 19–42 2 847.40 3 26.72
Protein Name Uniprot ID MW (Da) pI Protein size (aa) Coverage Mascot score
PPP1R2P3 IPP2M_HUMAN 23.048 4.82 205 7.3% 48.95
Peptide Range (start-end) Number of spectra m/z meas. z Mascot score
K.WDEINILATYHPADK.G 47–61 3 595.96 3 48.95
Protein Name MW (Da) pI Protein size (aa) Coverage Mascot score
Common to both PPP1R2 and PPP1R2P3 49.3% 546.34
Peptide Range (start-end) Number of spectra m/z meas. z Mascot score
R.KLAAAEGLEPK.Y 103–113 7 563.83 2 70.38
R.IQEQESSGEEDSDLSPEER.E 116–134 5 1082.46 2 117.82
K.LAAAEGLEPK.Y 104–113 5 499.78 2 63.21
K.LHYNEGLNIK.L 146–155 4 400.88 3 53.17
R.KLHYNEGLNIK.L 145–155 4 664.87 2 51.25
K.LHYNEGLNIKLAR.Q 146–158 2 514.29 3 23.57
R.IQEQESSGEEDSDLSPEEREK.K 116–136 2 807.69 3 59.32
K.DLHDDDEDEEMLETADGESMNTEESNQGSTPSDQQQNK.L 164–201 1 1434.23 3 30.63
K.YRIQEQESSGEEDSDLSPEER.E 114–134 1 828.70 3 11.42
K.LAAAEGLEPKYR.I 104–115 1 439.91 3 15.97
K.TSTTSSMVASAEQPR.G 19–33 1 784.86 2 49.60
Protein Name Uniprot ID MW (Da) pI Protein size (aa) Coverage Mascot score
PPP1CC2 PP1G_HUMAN 38.518 5.78 337 15% 252.57
Peptide Range (start-end) Number of spectra m/z meas. z Mascot score
R.VASGLNPSIQK.A 315–325 5 557.32 2 54.76
R.GVSFTFGAEVVAK.F 222–234 2 656.35 2 72.94
K.NVQLQENEIR.G 27–36 2 621.83 2 42.11
K.YPENFFLLR.G 114–122 1 599.82 2 39.6
K.LNIDSIIQR.L 7–15 1 536.31 2 43.16

Korrodi-Gregório et al.

Korrodi-Gregório et al. BMC Cell Biology 2013 14:15   doi:10.1186/1471-2121-14-15

Open Data