PPP1R2/PPP1R2P3 are present in human testis and sperm extracts. (A), PPP1R2/PPP1R2P3 and PPP1CC2 presence in testis and in the RIPA supernatant (soluble fraction) and pellet (insoluble fraction) of sperm lysates, was analyzed using sheep anti-PPP1R2 and rabbit anti-PPP1CC2 antibodies. 100 μg of both fractions were loaded in a SDS-PAGE gel. Acetylated α-tubulin was used as a loading control. (B), A human sperm sample (2 × 108 sperm cells) was sonicated to disrupt head and tail bond, and subjected to a sucrose gradient. Both pools were verified by phase contrast (PH). 100 μg of both fractions were loaded in a SDS-PAGE gel. After transfer, the blot was probed with sheep anti-PPP1R2 and rabbit anti-PPP1CC2 antibodies. Acetylated α-tubulin was used as a loading control.
Korrodi-Gregório et al. BMC Cell Biology 2013 14:15 doi:10.1186/1471-2121-14-15