Comparison of PPP1R2 and PPPR2P3 phosphorylation by GSK3 and CK2. Recombinant PPP1R2 and PPP1R2P3 were incubated in kinase buffer with reactive ATP (0); or in the presence of GSK3β kinase (G), CK2 kinase (C) or both kinases (G/C), for 90 min at 30oC. Protein phosphorylation was detected by autoradiography (32P) following SDS-PAGE. Immunoreactivity detected with a specific sheep anti-PPP1R2 antibody is shown for comparative purposes.
Korrodi-Gregório et al. BMC Cell Biology 2013 14:15 doi:10.1186/1471-2121-14-15