Figure 2.

Interaction of PPP1R2P3 with different PPP1 isoforms. (A), Sequential transformation of yeast AH109 with bait plasmid (pAS2-PPP1CA, pAS2-PPP1CC1, pAS2-PPP1CC2, or pAS2-PPP1CC2end) and the prey plasmid pACT2-PPP1R2P3. pAS2-PPP1CC2end is the unique C-terminal tail of PPP1CC2 produced by alternative splicing of the PPP1CC gene. For negative and positive controls pAS2-1/pACT-2 and pVA3-1/pTD1-1 vectors were used, respectively (B), Overlay assay of PPP1R2 and PPP1R2P3. Commercial PPP1R2 and His-PPP1R2P3 were separated by SDS-PAGE, transferred to nitrocellulose and overlaid with recombinant PPP1CC1 or PPP1CC2, as indicated. Western blotting was performed with the respective specific antibodies.

Korrodi-Greg√≥rio et al. BMC Cell Biology 2013 14:15   doi:10.1186/1471-2121-14-15
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