Prey constructs for the split ubiquitin assay. Proteins were fused as indicated to NubG. Topology of the fusion proteins in the ER membrane is shown. Interaction of Wbp1-CPLV with Ost1-Nub was used as positive control. Expression of Nub-Alg5 in the ER membrane was used as negative control for all bait constructs.
Harty and Römisch BMC Cell Biology 2013 14:14 doi:10.1186/1471-2121-14-14