Nimotuzumab impeded the EGF-EGFR interaction. A: Serum-starved cells either were treated with medium (control) or medium containing nimotuzumab for 90 min followed by treatment with hEGF (40 ng/ml) for 15 min. Protein level of MMP-9, EGFR, pEGFR, ERK, and pERK were assessed by Western blotting. GAPDH expression is also shown. B: Densitometric analysis of the expression of the same proteins relative to GAPDH expression. Error bars indicate mean ± SD; n = 3 experiments; *P < 0.05.
Huang et al. BMC Cell Biology 2013 14:13 doi:10.1186/1471-2121-14-13