IFN-α induces phosphorylation of PKC-δ in Daudi and U266 B lymphoma cells. Daudi (A) and U266 (B) cells were cultured with 250 U/ml IFN-α, followed by Western blotting using Abs specific for pan- or phospho (Thr505)-PKC-δ. Arrows indicate specific bands for pPKC-δ, PKC-δ, and Actin, respectively. †, non-specific band. Expression of pan- or phospho-PKC-δ relative to actin is shown. Each point represents mean ± SD from five independent experiments. *Significantly different (p < 0.05) from time zero.
Yanase et al. BMC Cell Biology 2012 13:7 doi:10.1186/1471-2121-13-7