Table 1

Palmitoylation does not affect the binding of agonists.

HEKOPRM1

HEKC170A


Relative Affinities


Morphine

KH (nM)

2.8 ± 0.56

2.2 ± 0.42

KL (nM)

155 ± 27

123 ± 31


Naloxone

KI (nM)

5.1 ± 0.71

6.2 ± 1.2


CTOP

KI (nM)

11 ± 1.4

9.5 ± 2.1


The affinities of receptor for ligands were determined in HEKOPRM1 and HEKC170A cells using an agonist binding assay. One-site (KI: naloxone and CTOP) or two-site (KH and KL: morphine) curve-fitting models were used in the analyses with GraphPad Prism 5.0. Data were analyzed by one-way ANOVA with post-hoc Dunnett's test. Standard deviations are provided, and "*" represents significant changes (p < 0.05).

Zheng et al. BMC Cell Biology 2012 13:6   doi:10.1186/1471-2121-13-6

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