Palmitoylation inhibitor impairs homodimerization and cholesterol association. (a) HEKOPRM1 or HEKC170A cells were treated with 50 μM 2-BP or vehicle for 12 h, and the cholesterol associated with receptor complex was measured. (b) HEK cells were transfected with CFPOPRM1 and YFPOPRM1 or transfected with CFPC170A and YFPC170A for 24 h. 50 μM 2-BP or vehicle was used to treat the cells for additional 12 h. FRET was then determined. Two-way ANOVA with Bonferroni's test (a) or student t-test (b) was used. Error bars and "*" represent the standard deviations and significant changes (p < 0.05, n > 3), respectively.
Zheng et al. BMC Cell Biology 2012 13:6 doi:10.1186/1471-2121-13-6