Rab11-FIP3 is a cell cycle-regulated phosphoprotein
1 Henry Wellcome Laboratory of Cell Biology, Institute of Molecular, Cell and Systems Biology, College of Medical, Veterinary and Life Sciences, Davidson Building, University of Glasgow, Glasgow G12 8QQ, Scotland, UK
2 Department of Cell and Developmental Biology, University of Colorado Anschutz Medical Campus, 12801 E. 17th Avenue, Aurora, CO 80045, USA
3 Department of Cell Biology and Physiology, University of Pittsburgh Medical School, Pittsburgh, PA 15260, USA
4 Riken Centre for Developmental Biology, 2-2-3 Minatojima-minamimachi, Chuo-ku Kobe 650-0047, Japan
BMC Cell Biology 2012, 13:4 doi:10.1186/1471-2121-13-4Published: 8 March 2012
Additional file 1:
Figure S1. HA-FIP3-4A does not modulate cytokinesis. HeLa cells on glass coverslips were transiently transfected with HA-FIP3, or HA-FIP3-4A as described. After 48 h, cells were fixed and cells expressing HA-tagged FIP3 identified by immunostaining as described. In parallel, microtubules (anti-tubulin) and DNA (DAPI) were also stained. The fraction of cells expressing each FIP3 species that were binucleate was then determined and is plotted graphically in panel A. The data shown are from a representative experiment: over 200 GFP-positive cells were counted per condition. The experiment was repeated three times with similar results. Inset shows an anti-HA immunoblot of cell lysates to verify broadly similar expression levels of each construct.
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Additional file 2:
Figure S2. GFP-FIP3 distribution in early and late telophase is not modulated by phosphorylation of S280, S347 or Ser 450. HeLa cells on glass coverslips were transiently transfected with GFP-FIP3 (pseudo-coloured green), or the indicated mutants as described. After 24 h, cells were fixed and immunostained with anti-tubulin (pseudo-coloured red) and the distribution of cells in telophase examined. Shown are cells in early or late telophase (cf. Figure 5). Data from a representative experiment, repeated 5 times are shown.
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