Immunofluorescence staining for DMP1 in tissue sections of ceramic scaffolds seeded with iPSC-TGF-beta1-RA-cells and cortical bone. (A) DAPI staining in a tissue section from a murine cortical bone; (B) Immunofluorescence staining for DMP1 in a tissue section of cortical bone; (C) Immunofluorescence staining in a tissue section in which primary antibody was omitted; (D) DAPI staining in a tissue section from a ceramic scaffold seeded with iPSC-TGF-beta1-RA-cells; (E) Immunofluorescence staining of DMP1 in a tissue section of a ceramic scaffold seeded with iPSC-TGF-beta1-RA-cells; (F) Overlay image of D and E indicating that DMP1 expressing cells colocalize with DAPI staining cells. Original magnifications 200X. The data confirm that iPSC-TGF-beta1-RA-cells proceed to osteocyte differentiation in ceramic scaffolds.
Li and Niyibizi BMC Cell Biology 2012 13:35 doi:10.1186/1471-2121-13-35