Amino acids 1-106 are necessary for TRPC6 channel activity. A. Full-length HA-tagged TRPC6 was expressed together with the indicated FLAG-tagged TRPC6 constructs in HEK 293T cells. Expression of FLAG- and HA-tagged protein was confirmed by Western blot of whole cell lysates (top and middle panels). The ability of the amino-terminal truncated TRPC6 protein to multimerize with full-length TRPC6 was confirmed by FLAG immunoprecipitation followed by western-blotting for HA-TRPC6 (bottom panel). Whole cell currents in HEK cells transiently transfected with wild-type TRPC6 (B) or TRPC6 107-931 (C) under basal conditions and upon stimulation with 10 μM OAG. D. Average normalized current density in response to OAG. One way ANOVA ; * p value < 0.05; n=8 cells.
Carrasquillo et al. BMC Cell Biology 2012 13:33 doi:10.1186/1471-2121-13-33