Localization of Mig2-GFP in Δmsn5 yeast cells. The FMY501(Mig2-GFP) and FMY535 (Δmsn5 Mig2-GFP) yeast strains, were grown in YEPD high-glucose medium (H-Glc) until an A600nm of 1.0 was reached and then transferred to YEPGly low glucose medium (L-Glc) for 5 min. Cells were stained with DAPI and imaged for GFP and DAPI fluorescence. Scale bar is 10 μm. The nuclear and mitochondrial localization of Mig2-GFP protein was determined in at least 100 cells per growth condition. No statistically significant differences were detected between the wild-type and the mutant strains. N, denotes a nuclear fluorescence signal and M, mitochondrial fluorescence signal.
Fernández-Cid et al. BMC Cell Biology 2012 13:31 doi:10.1186/1471-2121-13-31