Representative 2D FISH images displaying examples of peripheral, intermediately and internally positioned chromosome territories within (SOB), (ESK4), MSCs and lymphocytes. Whole chromosome painting probes were labelled with biotin and detected using streptavidin conjugated to Cy3 (pseudocoloured green) and the nuclei were counterstained with DAPI (blue). The number/letter on the top right hand-side of each image depicts the chromosome painted in each nucleus. Bar, 10 μm.The right hand panel demonstrates how the erosion analysis works by delineating the DAPI mask and then eroding in revealing five shells of equal area in which the % signal of DAPI and the chromosome signal are measured.
Foster et al. BMC Cell Biology 2012 13:30 doi:10.1186/1471-2121-13-30