Threonine phosphorylation of p68 in HCT116 cells under oxaliplatin (Oxa) treatment. (A) Apoptosis of HCT116 cells under the treatment of different concentrations of oxaliplatin is measured by a commercial apoptosis kit and presented as Relative apoptosis by defining the apoptosis of the cells without oxaliplatin treatment as 1. Error bars represent standard deviations of four independent experiments. (B), (C), and (D) Threonine phosphorylations of p68 in HCT116 cells that are treated with different concentrations of oxaliplatin (B) and (C) or 20 μM of oxaliplatin for different times (D) are analyzed by immunobloting the p68 that is immunoiprecipitated (IP:p68) from cell lysates using antibody against phorsphor-threonine (IB:14B3). Immunoblot of p68 (IB:p68) in immunoprecipitates indicate the amounts of p68 were precipitated down. In (C), the analyses are carried out with the cells that are already floating (Floating) and the cells that are still adherent (Adherent) under the treatments.
Dey and Liu BMC Cell Biology 2012 13:27 doi:10.1186/1471-2121-13-27