miR-980 is involved in stress response and miR-252 is required for muscle development. (a) Muscle degeneration assayed in wt and miR-980 mutants kept for 6 and 18 days at 25°C and 33°C. (b) Exemplary histological sections of control and miR-980 muscles after 18 d at 33°C. (c) High temperature (~38°C) mobility assay revealed no difference between wt and miR-980 mutants. (d) When housed at 25°C, miR-980 mutants did not have a shortened lifespan compared to control w1118/OregonR, but at 33°C their lifespan was significantly lower than in wild type animals. (e) Muscle degeneration assayed in young, old and stressed wt and miR-252 mutants. (f) Exemplary histological sections of control and miR-252 muscles from 3–4 d old flies. (g) High temperature (~38°C) mobility assay revealed that miR-252 mutants have mobility defects under hyperthermic stress. (h) When housed at 25°C and 33°C, miR-252 mutants did not have a shortened lifespan. Muscle degeneration was compared using the Chi-squared statistic where statistical significance of the other assays was done with the Student’s two-tailed t-test: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.
Marrone et al. BMC Cell Biology 2012 13:26 doi:10.1186/1471-2121-13-26