Dg-Dys-Syn1 regulates levels of miRNAs found in the nervous system and musculature. q-PCR analysis revealed the expression levels during all life stages and in adult body parts for (a) miR-956 (c) miR-980 and (e) miR-252. All levels have been normalized to those found in whole adult male flies. (b,d and f) LNA in situ hybridization: (b) miR-956 is present in the 3rd instar larval brain, eye discs and body wall muscles. (d) miR-980 is present in the 3rd instar larval brain in axons of photoreceptor cells in the optic lobe, in the eye disc, and in the midbrain. Note the reduced expression levels in miR-980 hypomorphs. miR-980 expression was not detected in the larval body wall muscles. (f) LNA in situ hybridization shows neuronal miR-252 in the optic lobe, eye disc, midbrain and ventral ganglion. Expression is not detected in the miR-252 mutant. miR-252 expression was not detected in larval body wall muscles. (g) RT q-PCR analysis of selected predicted target genes Dg, Dys, Syn1, robo and vimar in miR-252 amorphs and Dg in miR-980 hypomorphs. Three independent RNA extractions were conducted per genotype. Statistics were done using a two-tailed Student’s t-test: *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001.
Marrone et al. BMC Cell Biology 2012 13:26 doi:10.1186/1471-2121-13-26