(A) Examples of TMA scoring based on staining intensity. Light microscope images of mucinous ovarian tumour tissue (T) and stroma (S) from the TMAs. Tissue samples expressing sFRP4 are stained brown with DAB and counterstained with haematoxylin. Images show examples of (a) Negative (b) Weak (c) Moderate and (d) Strong staining. Scale bar = 30 μm. (B) Graphical representation of the staining intensity quantified for sFRP4 expression and β-catenin expression respectively, following histological quantification of the tumour cores into benign, weak, or strong. The y axis enumerates the staining intensity within each group of tumours.
Saran et al. BMC Cell Biology 2012 13:25 doi:10.1186/1471-2121-13-25