(A) Light microscope images (400X) of each cell line before and after Cisplatin treatment. sFRP4 protein stained brown with DAB and counterstained with haematoxylin for contrast (Scale bar = 50 μm). (B) Comparison of sFRP4 protein expression between untreated and treated cells of all four cell lines. (C) Quantification of the proportion of cells stained positive for sFRP4 following Cisplatin treatment in chemosensitive cell line A2780 vs. Chemoresistant cell lines ADR and Cis. Values represent the mean for each group ± SEM. (* p < 0.01; ** p < 0.001 one way ANOVA and LSD).
Saran et al. BMC Cell Biology 2012 13:25 doi:10.1186/1471-2121-13-25