Open Access Research article

Secreted frizzled-related protein 4 expression is positively associated with responsiveness to Cisplatin of ovarian cancer cell lines in vitro and with lower tumour grade in mucinous ovarian cancers

Uttara Saran1, Frank Arfuso1*, Nikolajs Zeps234 and Arunasalam Dharmarajan15

Author Affiliations

1 School of Anatomy and Human Biology, Faculty of Life and Physical Sciences, The University of Western Australia, 35 Stirling Highway, Perth, Crawley 6009, Western Australia

2 St John of God HealthCare, Subiaco, 6008, Western Australia

3 School of Surgery, The University of Western Australia, 35 Stirling Highway, Crawley, 6009, Western Australia

4 School of Pathology and Laboratory Medicine, The University of Western Australia, 35 Stirling Highway, Crawley, 6009, Western Australia

5 School of Biomedical Sciences, Faculty of Health Sciences, Curtin University and Curtin Health Innovation Research Institute (CHIRI), GPO Box U1987, Perth, 6845, Western Australia

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BMC Cell Biology 2012, 13:25  doi:10.1186/1471-2121-13-25

Published: 8 October 2012

Additional files

Additional file 1:

Figure S1. A representative image of a Western blot showing sFRP4 protein expression across the four cell lines.

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Additional file 2:

Figure S2. Representative images cut from Western blots demonstrating (A) sFRP4 protein expression was increased in the chemoresistant cell lines following transfection with sFRP4 plasmid; (B) sFRP4 mRNA expression of chemosensitive cell line A2780 was knocked down using siRNA; (C) sFRP4 protein expression of A2780 cells following siRNA treatment.

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Additional file 3:

Figure S3. Representative images cut from Western blots demonstrating (A) β-catenin protein expression across the three cell lines; (B) β-catenin protein expression in chemosensitive A2780 cells following siRNA treatment.

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