Rapid degradation of Hax-1 is dependent on its PEST sequence. A. Schematic representation of a PEST sequence in Hax-1 protein. The PEST sequence was identified using Pestfind service on “emboss.bioinformatics.nl/cgi-bin/emboss/pestfind”. The PEST sequence in Hax-1 is conserved among different mammals. B. Chase-time experiment of Hax-1 and other Bcl-2 proteins. H1299 cells treated with CHX (100 ug/ml) for different time points were harvested for immunoblot analysis using indicated antibodies. C. Data from three independent experiments in B were quantified. D. Similar experiments as B were carried out using mouse N2a cells. E. An EGFP-tagged WT Hax-1 or ΔPEST Hax-1 was transiently transfected into H1299 cells. Forty-eight hours later, CHX chase experiments were carried out. F. Quantitative analysis of data from E with three independent experiments.
Li et al. BMC Cell Biology 2012 13:20 doi:10.1186/1471-2121-13-20