Figure 2.

ERL-1 association into high MW complexes depends on Phe-303. Sucrose density gradient centrifugation was performed on HEK293 cells transiently transfected with wild type (upper panel) or F303A (lower panel) ERL-1HA. Twelve fractions were collected from each gradient, which were analyzed by Western blotting using an HA-tag specific antibody.

Hoegg et al. BMC Cell Biology 2012 13:2   doi:10.1186/1471-2121-13-2
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